Discovering human DNA replication initiation sites - paper published
May 09, 2025
Jamie and Rose’s paper, discovering the sites of DNA replication initiation across the human genome on single molecules, is published in Genome Biology. Discovering where DNA replication initiates in human cells is crucial for understanding how cells copy their genetic information. Our study explores where and how often DNA replication begins across the human genome. To achieve this, we employed a technique using in vivo BrdU incorporation followed by detection by single-molecule nanopore sequencing. We discovered that a large proportion of DNA replication begins outside known initiation zones, demonstrating a dispersed initiation pattern across the genome. Read more about our findings here or go straight to the article here.